csRNA-seq:
Technique that isolates 5' capped, short RNAs (20-60nt)
from total RNA to map initiating transcripts genome-wide.
Maps transcription activity at regulatory elements and
efficiently captures initiation from both promoters and
enhancer regions. (also works for
Start-seq/5'GRO-seq/GRO-cap/PRO-cap/TSS-seq/5'RNA-seq,
etc.)
ChIP-Seq:
(Tutorials 1-10 above are geared toward ChIP-Seq and
RNA-Seq) Isolation and sequencing of genomic DNA "bound"
by a specific transcription factor, covalently modified
histone, or other nuclear protein. This methodology
provides genome-wide maps of factor binding. Most of
HOMER's routines cater to the analysis of ChIP-Seq data.
RNA-Seq:
(This one is currently only a quick-recipe driven list of
commands, but the tutorials 1-3, & 8 above are geared
toward RNA-Seq) Extraction, fragmentation, and sequencing
of RNA. There are many variants on RNA-seq too, such as
Ribo-Seq (isolation of ribosomes translating RNA), small
RNA-Seq (to identify miRNAs), etc.
GRO-Seq: RNA-Seq of nascent
RNA. Transcription is halted, nuclei are isolated,
labeled nucleotides are added back, and transcription
briefly restarted resulting in labeled RNA
molecules. These newly created, nascent RNAs are
isolated and sequenced to reveal "rates of transcription"
as opposed to the total number of stable transcripts
measured by normal RNA-seq.
Hi-C:
Genomic interaction assay for understanding genome 3D
structure. This assay is much more specialized - For
more information about how to use HOMER to analyze Hi-C
data, check out the
Hi-C
analysis section.
DNase-Seq: Treatment of nuclei with
a restriction enzyme such as DNase I will result in
cleavage of DNA at accessible regions. Isolation of
these regions and their detection by sequencing allows the
creation of DNase hypersensitivity maps, providing
information about which regulatory elements are accessible
in the genome.